Molluscan Neuroscience Resources

In this section users can share protocols and techniques for Molluscan Neuroscience.  They can also share files and other resources.  Registered users can create pages to provide illustrated details on resources of common interest. To create a new page, click "Add child page".  To edit an existing page, click on that page and then click on the "edit" tab.  Edit the Body of the page using the editing box.

Genome resources for molluscan neurobiologists

The attached pdf has instructions from Wayne Sossin on using BASH and other tips for making use of genomic information for Aplysia research.

Lottia genome site  –http://genome.jgi-psf.org/Lotgi1/Lotgi1.home.html

NCBI WEB site:        http://www.ncbi.nlm.nih.gov/blast/Blast.cgi

The access to the Aplysia (whole genome shotgun) WGS database has changed.  You may still be able to use the old link below, but there may be difficulties.  The current access that is recommended as of Feb, 2011 is:

http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PAGE=MegaB...

Why the site for the WGS blast has changed is unclear.

(Tom Abrams)


Sossin trace archive tblastn site      http://grsites.com/aplysia/

Trace Archive NCB site 

http://www.ncbi.nlm.nih.gov/blast/Blast.cgi?PAGE=Nucleotides&PROGRAM=bla...

Broad Institute Aplysia page        http://www.broad.mit.edu/node/435

  Email from Jessica Alfoldi at Broad Institute on Jan 25, 2011

Hello everyone,
I just wanted to give you all an update on the Aplysia genome project.
As I'm sure you know, none of us have been terribly happy about the
state of the Aplysia genome assembly. The Broad Institute recently
sequenced some Aplysia BACs derived from sperm and concluded from
comparing that sequence to the genome assembly that difficulty with
the assembly derived from a extremely high level of transposons and
simple sequence repeats. We are beginning a new, all-Illumina genome
sequencing project in the hopes that the lack of dependence on
bacterial cloning will result in an improved genome assembly. We will
also be doing a parallel RNA-seq project using a panel of RNAs
provided by Leonid Moroz.
I'll keep you posted on the status of this project as we pass our
various milestones.
Best,
Jessica Alfoldi
AttachmentSize
genome.pdf5.66 MB

Molluscan Links

This is a list of Websites that may be of interest to other molluscan neuroscientists. 

Please add your favorites here by clicking on the edit tab.

Molluscan Neuroscience Laboratories

This is a list of primary investigators who work on Molluscan Neuroscience. 

Please add your lab to this list by clicking on the edit tab.